Baculovirus Production Services
Baculovirus Production Services
The baculovirus expression vector system (BEVS), first described in the 1980s, has proven successful for the production of many recombinant proteins. In principle, any foreign gene may be expressed in the BEVS for producing proteins in insect cells (IC). Protein therapeutics such as enzymes, tissue plasminogen activator (tPA), and viral and parasitic proteins have been produced using the BEVS-IC. Some viral structural proteins have been proven to self-assemble into ordered structures such as virus-like particles (VLP) during production in BEVS. These virus-like particles are commonly remarkably immunogenic and therefore potentially useful as vaccines. The baculovirus expression system is also promising for delivering genes into mammalian cells for gene therapy and other diverse applications.
Some unique features of the baculovirus system make this virus very appealing for preventive or therapeutic gene transfer. The system offers several advantages, including:
- Able to perform complex post-translational modifications (PTMs)
- High success rate of soluble protein recovery
- Generate large amounts of recombinant protein
- Can express genes from viruses, bacteria, plants and mammals at levels from 1-500mg/l
QVirusTM Platform can provide highly effective baculovirus expression vector construction services to support protein production. Our custom baculovirus construction services include:
- Gene synthesis or subcloning of customer’s DNA into the baculovirus transfer vector
- Generation of recombinant bacmid DNA
- Transfection of insect cells with bacmid DNA
- Generation of P1 stock (low titer)
- Validation by Western blot
- Amplification of high titer recombinant baculovirus stock (P2)
- Small scale culture to test the expression level and subsequent optimization, if necessary
Highlights
- Advanced optimization system — we provide advanced codon optimization specific to the Sf21, the Sf9, the Hi-5 insect cell lines based on the nature of the protein.
- One-stop service — we can cover all aspects of your project from gene synthesis, cloning, virus packaging and virus amplification.
- Value — we focus on the quality of our service and all supported by competitive pricing and fastest turnaround time.
QVirusTM Platform offers custom baculovirus subcloning and virus production, allowing high-level gene expression in insect cells. If you have any special requirements, please feel free to contact us.
Reference
1. Contreras-Gómez, A, et al. Protein production using the baculovirus-insect cell expression system. Biotechnology Progress, 2014, 30(1):1-18.
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