Adenovirus Production Services

Adenovirus is a replication-defective adenoviral vector system that widely used for gene delivery in a variety of cell types. The vectors are predominantly nonenveloped, nonintegrating, episomal, double-stranded DNA viruses. With a strong safety record, adenoviruses and recombinant adenoviral vectors have been widely used for gene function studies and human gene therapy. Recombinant adenoviruses that cannot replicate are attractive vectors for gene delivery for the following reasons:

1. Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues.
2. Recombinant adenoviruses do not disrupt the genome of the host cell but remain in the nucleus as episomal DNA.
3. Recombinant adenoviruses provide transient, high-level gene expression.
4. Great packaging capacity, up to 8kb.
5. Recombinant adenoviruses are easily amplified and purified to high concentrations (1x1010pfu/mL-1x1011pfu/mL).
6. Recombinant adenoviruses are suitable for both in vitro and in vivo applications.

Three generations of adenoviral vectors in gene therapy.

Figure 1. Three generations of adenoviral vectors in gene therapy.

QVirus™ Platform has launched a comprehensive adenovirus production service, including a variety of different adenoviral expression systems used for gene over-expression and shRNA mediated gene down-regulation, which may also allow researchers to produce adenoviruses by themselves as they need.

Adenovirus Construction, Generation and Amplification Options

  • Cloning the gene of interest or shRNA into the adenoviral genome;
  • Producing the primary, low-titer virus stock;
  • Amplifying the adenovirus according to your research needs;
  • Adenovirus purification and titration (qPCR titration or PFU titration);
  • Technical support for adenovirus using.

QVirus™ Platform's Benefits

➢ Large promoter and marker gene portfolio guarantees for flexibility in vector design
➢ Multiple cloning systems: E1 and E3 deletion, E1 intact and E3 deletion, E1 deletion and E3 intact
➢ Up to four genes from one vector through large packaging capacity
➢ Produce high titer adenovirus particles suitable for in vitro cell tests or in vivo animal experiments
➢ Typically in a few of weeks to save your precious time for research reproducible results due to standardized product quality

Adenovirus packaging by QVirus™ Platform shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vitro and in vivo. Therefore, we have a growing base of highly satisfied customers who come back to us time after time for their adenoviral vector cloning and packaging needs. If you have any special requirements, please feel free to contact us.

Reference
1. Lee C S, et al. Adenovirus-Mediated Gene Delivery: Potential Applications for Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes & Diseases, 2017:S2352304217300272.

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