Lentiviral expression vectors are the most effective tools for transducing and stably expressing different effector molecules (cDNA, DNA fragments, siRNA, antisense, ribozymes, etc.) or reporter constructs in virtually any mammalian cell, including nondividing cells and whole model organisms. Like standard plasmid vectors, it is able to introduce lentiviral constructs in plasmid form into the cells with low-to-medium efficiency and get transient expression of effectors by conventional transfection protocols. Through packaging the lentiviral expression construct into pseudoviral particles, we can obtain highly efficient transduction, even with the most difficult to transfect cells, such as primary, stem, and differentiated cells. Recombinant lentiviral vectors (rLV) are widely used due to the following features:
Nevertheless, production of recombinant lentiviral vectors is hard and challenging, especially when high titer of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) are required. Through the QVirus™ platform at Creative Biogene, we have been generated more lentiviruses than other popular systems. Lentiviruses generated from QVirus™ platform have high safety profile and high efficiency of gene delivery to almost all cell types and whole model organisms.
1) Synthesize your genetic materials (cDNA, miRNA, shRNA, etc.)
2) Lentivirus plasmid vector construction;
3) Lentivirus packaging;
4) Lentivirus purification;
5) Lentivirus tittering.
|Regular Titer||>107||Standard cell culture models|
|High Titer||>108||More difficult-to-transduce cells, such as suspension cells|
|Ultra-high Titer||>109||Very difficult-to-transduce cells, such as stem cells and primary cells;|
In vivo applications
Our lentivirus production team has constructed, packaged, and purified thousands of lentiviruses from small crude scale to large purified scale for worldwide clients in more than a decade. QVirus™ Platform has always optimized our lentivirus production systems, including lentivirus plasmid construction and low endotoxin plasmid preparation, cell culture medium, host cells for lentivirus packaging and lentivirus packaging process, small and large scale lentivirus purification process. If you have any special requirements, please feel free to contact us.