Custom Lentivirus Production Service

Lentiviral expression vectors are the most effective tools for transducing and stably expressing different effector molecules (cDNA, DNA fragments, siRNA, antisense, ribozymes, etc.) or reporter constructs in virtually any mammalian cell, including nondividing cells and whole model organisms. Like standard plasmid vectors, it is able to introduce lentiviral constructs in plasmid form into the cells with low-to-medium efficiency and get transient expression of effectors by conventional transfection protocols. Through packaging the lentiviral expression construct into pseudoviral particles, we can obtain highly efficient transduction, even with the most difficult to transfect cells, such as primary, stem, and differentiated cells. Recombinant lentiviral vectors (rLV) are widely used due to the following features:

  • Lentiviral vectors can accommodate long sequences;
  • Lentiviruses seem to be non-immunogenic due to lack of viral coding sequence transfer;
  • Lentiviruses are able to transduce non-dividing cells;
  • Proteins can be stably expressed because of integration into the cell chromosome.

Nevertheless, production of recombinant lentiviral vectors is hard and challenging, especially when high titer of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) are required. Through the QVirus™ platform at Creative Biogene, we have been generated more lentiviruses than other popular systems. Lentiviruses generated from QVirus™ platform have high safety profile and high efficiency of gene delivery to almost all cell types and whole model organisms.

Our Complete Custom Lentivirus Production Services

1) Synthesize your genetic materials (cDNA, miRNA, shRNA, etc.)
2) Lentivirus plasmid vector construction;
3) Lentivirus packaging;
4) Lentivirus purification;
5) Lentivirus tittering.

Available titer options

TiterTU/mlApplication
Regular Titer>107Standard cell culture models
High Titer>108More difficult-to-transduce cells, such as suspension cells
Ultra-high Titer>109Very difficult-to-transduce cells, such as stem cells and primary cells;
In vivo applications

Applications of Custom Lentivirus Production Service

  • Gene transfer and genome editing for expression or silencing of a gene
  • Lentiviral based stable cell line generation
  • Knock-out and knock-in cell line generation with CRISPR genome editing
  • Chimeric Antigen Receptor T (CAR T)-cell engineering with AAV or alone
  • Production of transgenic animals
  • Gene therapy studies

Features

  • Provide custom construction of lentivirus expression plasmid with different promoters or tags
  • Consistent and reliable titers delivered at or above specified titer levels
  • Three different titer levels and flexible production scales
  • High titer for animal injection use, up to 10^10 TU/mL
  • Reduced risk of insertional mutagenesis
  • Time and cost-saving

Our lentivirus production team has constructed, packaged, and purified thousands of lentiviruses from small crude scale to large purified scale for worldwide clients in more than a decade. QVirus™ Platform has always optimized our lentivirus production systems, including lentivirus plasmid construction and low endotoxin plasmid preparation, cell culture medium, host cells for lentivirus packaging and lentivirus packaging process, small and large scale lentivirus purification process. If you have any special requirements, please feel free to contact us.

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